Youinou P, Mackenzie L, Bourbigot B, Le Saout J, Le Goff P, Jouquan J, Lydyard P M
Rev Rhum Mal Osteoartic. 1986 Nov;53(11):643-7.
Molecules recognised by the monoclonal antibodies Leu 1 - OKT 1 - Mid 5 (CD5 molecules) were originally shown on human T cells and are equivalent to Lyt-1 molecules in the mouse. These surface molecules are detectable on some human B cells, but are weakly expressed. We have therefore tested the effects of the tumour-promoter, phorbol myristic acetate (PMA) on expression of these molecules on B cells. Sheep red blood cell rosette-depleted lymphoid organ or blood cells from 8 controls and 10 rheumatoid arthritis (RA) patients were examined fresh and cultured with PMA for 48 hours and the cells stained with Leu 1 or Mid 5 by indirect immunofluorescence. Cells were analysed by UV microscopy or flow cytometry. More RA blood B cells were positive for Leu 1 than normals before culture. Following incubation with PMA, the percentage of positive cells increased in all cell populations (1-49% in RA patients and 5-26% in controls). There was no difference in the expression of Leu 1 on Kappa--or lambda--positive cells before and after culture in PMA. Leu 1 positive cells were under-represented in Epstein-Barr virus-driven cell lines.
单克隆抗体Leu 1 - OKT 1 - Mid 5所识别的分子（CD5分子）最初在人T细胞上被发现，相当于小鼠中的Lyt-1分子。这些表面分子在一些人B细胞上可检测到，但表达较弱。因此，我们测试了肿瘤促进剂佛波醇肉豆蔻酸酯（PMA）对这些分子在B细胞上表达的影响。对来自8名对照者和10名类风湿性关节炎（RA）患者的去除绵羊红细胞花环的淋巴器官或血细胞进行新鲜检查，并与PMA一起培养48小时，然后通过间接免疫荧光用Leu 1或Mid 5对细胞进行染色。通过紫外显微镜或流式细胞术对细胞进行分析。培养前，RA血液B细胞中Leu 1阳性的细胞比正常人多。与PMA孵育后，所有细胞群体中阳性细胞的百分比均增加（RA患者中为1 - 49%，对照者中为5 - 26%）。在PMA中培养前后，κ或λ阳性细胞上Leu 1的表达没有差异。在爱泼斯坦 - 巴尔病毒驱动的细胞系中，Leu 1阳性细胞占比不足。
