[肾综合征出血热病毒株在细胞培养中的分离]

[Isolation of strains of the virus of hemorrhagic fever with renal syndrome in cell culture].

作者信息

Bashkirtsev V N, Tkachenko E A, Dzagurova T K, Ryl'tseva E V

出版信息

Vopr Virusol. 1984 Jul-Aug;29(4):497-502.

Abstract

Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were isolated from the lungs of Cl. glareolus caught in natural foci of HFRS in the European USSR. Preliminary experiments using enzyme-immunoassay (EIA) established the appurtenance of HFRS antigen in lung suspensions to the "western" serotype. Four strains were isolated in Vero-E6 cell culture after several blind passages and identified as the "western" serotype of HFRS virus. One stram originally isolated in Wistar rats and passaged in them 4 times and then adapted to Vero-E6 cells showed a closer antigenic relationship with the "eastern" HFRS virus serotype.

摘要

从苏联欧洲部分肾综合征出血热（HFRS）自然疫源地捕获的棕背䶄肺脏中分离出4株HFRS病毒。采用酶免疫测定法（EIA）进行的初步实验确定了肺悬液中的HFRS抗原属于“西方”血清型。经过多次盲传后，在Vero-E6细胞培养物中分离出4株病毒，并鉴定为HFRS病毒的“西方”血清型。最初在Wistar大鼠中分离出并在其体内传代4次，然后适应Vero-E6细胞的1株病毒与“东方”HFRS病毒血清型显示出更密切的抗原关系。

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