Ivanov A P, Tkachenko E A, Petrov V A, Pashkov A J, Dzagurova T K, Vladimirova T P, Voronkova G M, van der Groen G
Institute of Poliomyelitis and Virus Encephalitides of the U.S.S.R. Academy of Medical Sciences, Moscow.
Arch Virol. 1988;100(1-2):1-7. doi: 10.1007/BF01310902.
Consecutive serum samples collected from 235 patients with Haemorrhagic Fever with Renal Syndrome (HFRS), between two days and two years after onset of disease, have been analysed for the presence of IgG and IgM type of antibodies specific for Hanta-viruses. The sera were screened in parallel by a newly developed indirect Immuno Enzyme Assay (EIA) in parallel with Indirect Immunofluorescent Antibody Assay (IFA). In both tests the Hantaan virus strain 76-118 was used as the antigen. The EIA was much more sensitive than the IFA test for the detection of IgM type antibodies. With the indirect EIA IgM type antibodies against Hantaan virus 76-118 have been detected in HFRS patient's sera from the second day of illness indicating the usefulness of this test for the early serological diagnosis of this disease.
对235例肾综合征出血热（HFRS）患者在发病后两天至两年期间采集的连续血清样本进行了分析，以检测汉坦病毒特异性IgG和IgM型抗体的存在。通过新开发的间接免疫酶测定法（EIA）与间接免疫荧光抗体测定法（IFA）对血清进行平行筛查。在两项检测中均使用汉滩病毒株76-118作为抗原。在检测IgM型抗体方面，EIA比IFA检测更为灵敏。通过间接EIA，在发病第二天的HFRS患者血清中检测到了针对汉滩病毒76-118的IgM型抗体，表明该检测方法对该病的早期血清学诊断有用。
