Vigo J, Lahmy S, Salmon J M, Viallet P
C R Acad Sci III. 1984;299(12):489-94.
Under pulsed excitation the decay curve of the intrinsic cellular fluorescence has been recorded using an original equipment. Cell by cell experiments are feasible so that the NAD(P)H bound/free ratio and the relaxation time characteristic of bound NAD(P)H can be used in order to build up histograms characterizing the cell population.