Bhakdi S, Roth M, Nürnberger W
J Immunol Methods. 1984 Nov 16;74(1):79-86. doi: 10.1016/0022-1759(84)90369-7.
We describe a simple and reliable method for quantitating C3d in human plasma. The method rests on the finding that native C3 and its activation/inactivation product C3c bind to Concanavalin A, whereas C3d does not. Rocket affinoimmunoelectrophoresis with Con-A Sepharose incorporated into an intermediate gel permits quantitation of free C3d in 2-20 microliters aliquots of EDTA-plasma without any manipulation prior to sample application. Using this method, we found that the level of circulating C3d in plasma of 30 healthy donors was usually well below 3% of maximally convertable C3d.
我们描述了一种用于定量人血浆中C3d的简单可靠方法。该方法基于以下发现：天然C3及其激活/失活产物C3c与伴刀豆球蛋白A结合，而C3d不结合。将伴刀豆球蛋白A琼脂糖掺入中间凝胶的火箭免疫亲和电泳允许对2-20微升等分的EDTA血浆中的游离C3d进行定量，在加样前无需任何处理。使用该方法，我们发现30名健康供体血浆中循环C3d的水平通常远低于最大可转化C3d的3%。
