Becquemin J P, Benhaiem N, Gaston A, Geschwind H, Teisseire D, Boussignac H, Mellière D
J Mal Vasc. 1984;9(3):195-9.
Effects of laser radiation on atherosclerotic plaques were evaluated in vitro and in vivo. Necropsy artherosclerotic femoral and coronary arteries were submitted to a laser beam. Arteries were perfused by saline or blood solution and a silicone fibro-optic system was placed near the plaques through a balloon dilatation catheter. After laser radiation (20 to 40 W, 2 to 10 seconds) angiograms and histologic sections were obtained. Aorta of 20 atherosclerotic New Zealand rabbits was exposed in dry condition to a CO2 laser beam (2-4 W, 0,2 second). After reinstatement of blood flow, animals were sacrificed from 24 hours to 3 weeks for microscopical examination. In vitro studies showed a significant recanalisation of the thrombosed arteries under blood flow but not under saline solution. Histologically the laser radiation produced a crater surrounded by thermal intimal damage and decollated calcified plaques. In vivo studies showed the healing process: no perforation, no aneurysm, no thrombosis occurred. After 24 hours the crater was filled with a non extensive thrombus, on the first week the thrombus contained phagocyte cells and on the third week, the endothelial damage was completely healed. These results suggest that laser radiation may be effective to recanalize totally occluded arteries in human.
在体外和体内评估了激光辐射对动脉粥样硬化斑块的影响。对尸检获得的股动脉和冠状动脉粥样硬化标本施加激光束。用盐水或血液溶液灌注动脉，并通过球囊扩张导管将硅树脂光纤系统放置在斑块附近。在激光辐射（20至40瓦，2至10秒）后，获取血管造影照片和组织学切片。将20只患有动脉粥样硬化的新西兰兔的主动脉在干燥条件下暴露于CO2激光束（2 - 4瓦，0.2秒）。恢复血流后，在24小时至3周内处死动物进行显微镜检查。体外研究表明，在血流状态下血栓形成的动脉有明显的再通，但在盐溶液灌注下则没有。组织学上，激光辐射产生了一个火山口样区域，周围伴有热内膜损伤和剥落的钙化斑块。体内研究显示了愈合过程：未发生穿孔、动脉瘤和血栓形成。24小时后，火山口样区域充满了少量血栓，第一周时血栓中含有吞噬细胞，第三周时，内皮损伤完全愈合。这些结果表明，激光辐射可能对人类完全闭塞的动脉再通有效。
