Ando M, Sugimoto M, Nishi R, Suga M, Horio S, Kohrogi H, Shimazu K, Araki S
Thorax. 1984 Nov;39(11):850-6. doi: 10.1136/thx.39.11.850.
Pulmonary alveolar macrophages were obtained by saline lavage from 23 healthy male volunteers--10 non-smokers and 13 cigarette smokers. Lavage produced three times as many alveolar macrophages in smokers than in non-smokers. When macrophages from smokers and from non-smokers were incubated in vitro, more cells from smokers adhered to glass, spread out, and showed enhanced nitroblue tetrazolium (NBT) reduction. The surface morphology of alveolar macrophages from smokers showed more with a plate like appearance and ridge like membrane surface, while the macrophages from non-smokers were predominantly spherical with ruffles. The proportions of cells which stained highly for beta galactosidase were 55% in smokers and 11% in non-smokers. Thus, in a resting state in vitro, alveolar macrophages from smokers were more active than those from non-smokers. When, however, macrophages from smokers and non-smokers were incubated with immunobeads and with opsonised or non-opsonised BCG, the phagocytic activity and stimulated NBT reduction of alveolar macrophages from smokers were similar to or somewhat less than those of non-smokers.
通过盐水灌洗从23名健康男性志愿者（10名不吸烟者和13名吸烟者）获取肺泡巨噬细胞。灌洗产生的吸烟者肺泡巨噬细胞数量是不吸烟者的三倍。当吸烟者和不吸烟者的巨噬细胞在体外培养时，吸烟者的更多细胞黏附于玻璃上，铺展并显示出增强的硝基蓝四氮唑（NBT）还原能力。吸烟者肺泡巨噬细胞的表面形态更多呈现板状外观和嵴状膜表面，而不吸烟者的巨噬细胞主要呈球形且有褶皱。β-半乳糖苷酶染色强阳性的细胞比例在吸烟者中为55%，在不吸烟者中为11%。因此，在体外静息状态下，吸烟者的肺泡巨噬细胞比不吸烟者的更活跃。然而，当吸烟者和不吸烟者的巨噬细胞与免疫磁珠以及调理或未调理的卡介苗一起培养时，吸烟者肺泡巨噬细胞的吞噬活性和刺激后的NBT还原能力与不吸烟者相似或略低于不吸烟者。
