Morimoto Y, Kido M, Tanaka I, Fujino A, Higashi T, Yokosaki Y
Division of Respiratory Disease, University of Occupational and Environmental Health, Japan.
Br J Ind Med. 1993 Oct;50(10):955-60. doi: 10.1136/oem.50.10.955.
The objective of this study was to evaluate the combined effects of mineral fibres and cigarette smoke on the production of tumour necrosis factor (TNF) by alveolar macrophages. Rats were exposed to cigarette smoke in vivo, and production of TNF by alveolar macrophages was measured in the presence of mineral fibres in vitro. For smoke exposure, rats were divided into two groups. Five were exposed to a daily concentration of 10 mg/m3 of cigarette smoke for an eight hour period, and five rats (controls) were not exposed to smoke. Bronchoalveolar lavage was performed after exposure to smoke and the recovered alveolar macrophages were incubated with either chrysotile or ceramic fibres on a microplate for 24 hours. Activity of TNF in the supernatant was determined by the L-929 fibroblast cell bioassay. When alveolar macrophages were not stimulated by mineral fibres, production of TNF by rats exposed to smoke and unexposed rats was essentially the same. When alveolar macrophages were stimulated in vitro by chrysotile or ceramic fibres, production of TNF by alveolar macrophages from rats exposed to smoke was higher than that by alveolar macrophages from unexposed rats. The findings suggest that cigarette smoke and mineral fibres have a synergistic effect on TNF production by alveolar macrophages.
本研究的目的是评估矿物纤维和香烟烟雾对肺泡巨噬细胞产生肿瘤坏死因子（TNF）的联合作用。将大鼠在体内暴露于香烟烟雾中，并在体外有矿物纤维存在的情况下测量肺泡巨噬细胞产生TNF的情况。对于烟雾暴露，将大鼠分为两组。五只大鼠每天暴露于浓度为10mg/m³的香烟烟雾中8小时，另外五只大鼠（对照组）不暴露于烟雾。在暴露于烟雾后进行支气管肺泡灌洗，回收的肺泡巨噬细胞与温石棉或陶瓷纤维在微孔板上孵育24小时。通过L-929成纤维细胞生物测定法测定上清液中TNF的活性。当肺泡巨噬细胞未受到矿物纤维刺激时，暴露于烟雾的大鼠和未暴露大鼠产生TNF的情况基本相同。当肺泡巨噬细胞在体外受到温石棉或陶瓷纤维刺激时，暴露于烟雾的大鼠的肺泡巨噬细胞产生TNF的量高于未暴露大鼠的肺泡巨噬细胞。这些发现表明，香烟烟雾和矿物纤维对肺泡巨噬细胞产生TNF具有协同作用。
