Herrmann H, Günther E
Mol Gen Genet. 1984;197(2):286-91. doi: 10.1007/BF00330975.
After NG mutagenesis an FP2 donor was isolated which exhibited an enhanced conjugational capacity for chromosomal genes. The recombination frequency was increased by two orders of magnitude as compared to the parental strain. In plate matings recombinants arose at a frequency up to 5 X 10(-1) per donor cell. Late markers also recombined efficiently. An Hfr state of the donor strain was supported by (i) the high recombination frequency, (ii) the incompatibility reaction with plasmid pRO271 (= FP2::Tn401) and (iii) the clearcut transfer kinetics in interrupted matings, even for a late marker.
在进行NG诱变后，分离出了一个FP2供体，它对染色体基因表现出增强的接合能力。与亲本菌株相比，重组频率提高了两个数量级。在平板交配中，重组体的出现频率高达每个供体细胞5×10⁻¹。晚期标记也能有效地重组。供体菌株的Hfr状态得到以下几点支持：(i) 高重组频率；(ii) 与质粒pRO271（=FP2::Tn401）的不相容反应；(iii) 即使对于晚期标记，在中断交配中也有明确的转移动力学。
