Svrcek S, Ondrejka R, Mlynarcíková K, Svec J
Vet Med (Praha). 1984 Nov;29(11):643-52.
The trials were conducted within the full-scale research on the ecology of lyssa virus. In a period of the mass outbreak of common hamster population in the East Slovakian region, 283 hamsters were examined for rabies. Using the direct immunofluorescence method (DIFM), the rabies antigen was detected in the brain of five hamsters. Three virus strains (denoted as 3 O, 7 E, 9 E) were isolated by means of the inoculation test on sucking mice. On the basis of the detection of the nucleo-protein antigen by DIFM, or its inhibition, detection of the Babes-Negri bodies, determination of the neutralization index, titration on mice and determination of incubation time, the isolated strains were identified as the street strains of rabies virus. As determined by further detailed studies on biological characteristics (determination of the invasiveness index on animals with different susceptibility to rabies virus, determination of pathogenicity for different species of laboratory animals, different weight categories, with different methods of administration, invasiveness index), the "hamster" strains are included among those of intermediate virulence or reduced virulence. At intramuscular administration, the most virulent of the three "hamster" strains studied (3 O) induces a fatal course of rabies in common fox and cat; for wolves, dogs and rabbits it is apathogenic. This strain is also contained in the salivary glands of foxes and cats. In immunofluorescent detection of the rabies nucleoprotein antigen, the "hamster" strains formed a mixed picture of fluorescing particles, characteristic of street strains.
这些试验是在狂犬病病毒生态学的全面研究中进行的。在东斯洛伐克地区普通仓鼠种群大规模爆发期间，对283只仓鼠进行了狂犬病检测。采用直接免疫荧光法（DIFM），在5只仓鼠的大脑中检测到了狂犬病抗原。通过对乳鼠接种试验分离出3株病毒（分别记为3 O、7 E、9 E）。根据DIFM检测核蛋白抗原或其抑制情况、检测内基小体、测定中和指数、在小鼠上滴定以及测定潜伏期，将分离出的毒株鉴定为狂犬病病毒的街毒株。通过对生物学特性的进一步详细研究（测定对狂犬病病毒易感性不同的动物的侵袭指数、测定对不同种类实验动物、不同体重类别、不同给药方法的致病性、侵袭指数）确定，“仓鼠”毒株属于中等毒力或毒力降低的毒株。肌肉注射时，所研究的3株“仓鼠”毒株中最具毒力的毒株（3 O）可使普通狐狸和猫感染狂犬病并致死；对狼、狗和兔子则无致病性。该毒株也存在于狐狸和猫的唾液腺中。在狂犬病核蛋白抗原的免疫荧光检测中，“仓鼠”毒株形成了荧光颗粒的混合图像，这是街毒株的特征。
