Gutteridge J M
Biochem J. 1984 Dec 15;224(3):697-701. doi: 10.1042/bj2240697.
The stimulatory effect of ferrous salts on the peroxidation of phospholipids can be enhanced by EDTA when the concentration of Fe2+ in the reaction is greater than that of EDTA. Hydroxyl-radical scavengers do not inhibit peroxidation until the concentrations of Fe2+ and EDTA in the reaction are equal. Lipid peroxidation is then substantially initiated by hydroxyl radicals derived from a Fenton-type reaction requiring hydrogen peroxide. Superoxide radicals appear to play some role in the formation of initiating species.
当反应中Fe2+的浓度大于EDTA的浓度时,EDTA可增强亚铁盐对磷脂过氧化的刺激作用。在反应中Fe2+和EDTA的浓度相等之前,羟基自由基清除剂不会抑制过氧化反应。然后,脂质过氧化主要由源自需要过氧化氢的芬顿型反应的羟基自由基引发。超氧自由基似乎在引发物种的形成中起一定作用。