Searle A J, Willson R L
Biochem J. 1983 Jun 15;212(3):549-54. doi: 10.1042/bj2120549.
The stimulation of microsomal lipid peroxidation by FeSO4 and cysteine has been investigated. Although both FeSO4 and cysteine alone promoted an increase in malonaldehyde production, when these agents were added together to microsomes the resultant level of malonaldehyde was greater than the sum of the amounts formed by these pro-oxidants when acting individually. A further indication of an interaction between FeSO4 and cysteine was shown by the inhibitory action of chelating agents. Stimulation of peroxidation was shown to be independent of microsomal protein, including cytochrome P-450. The system has been characterized for the effects of cysteine, Fe2+ and O2 concentrations, pH, temperature and antioxidants. The results indicate that the high level of peroxidation attained with this system, its non-enzymic character and the involvement of hydroxyl radicals make it particularly useful for the investigation of the action of antioxidants. Furthermore it may also be a model of way in which decompartmentalized, delocalized or 'free' iron initiates peroxidation in vivo.
已对硫酸亚铁（FeSO4）和半胱氨酸对微粒体脂质过氧化的刺激作用进行了研究。尽管单独的FeSO4和半胱氨酸都能促进丙二醛生成量的增加，但当将这些试剂一起添加到微粒体中时，丙二醛的最终水平高于这些促氧化剂单独作用时形成的量之和。螯合剂的抑制作用进一步表明了FeSO4和半胱氨酸之间的相互作用。过氧化的刺激作用被证明与微粒体蛋白无关，包括细胞色素P - 450。该系统已针对半胱氨酸、Fe2+和O2浓度、pH、温度及抗氧化剂的影响进行了表征。结果表明，该系统达到的高水平过氧化、其非酶性质以及羟基自由基的参与使其对于研究抗氧化剂的作用特别有用。此外，它也可能是体内非分隔化、离域化或“游离”铁引发过氧化的一种方式的模型。
