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[犊牛定量免疫球蛋白测定的批判性评估]

[Critical evaluation of quantitative immunoglobulin determination in the calf].

作者信息

Lambrecht G, Petzoldt K

出版信息

Tierarztl Prax. 1984;12(4):535-9.

PMID:6442017
Abstract

From 3 calves fed with a single limited amount of colostrum 68 blood serum samples were tested comparatively by single radial immunodiffusion (RID) and by nephelometry for their IgG1-, IgA- and IgM-content. In comparison with nephelometry the commercial available RID kits seemed to be insufficient due to deficient agarose plates, difficulties in diluting, and weak reference sera. The results obtained by RID, therefore, were not comparable with those obtained by nephelometry which should be optimized, too.

摘要

从3头仅喂食少量初乳的小牛身上采集了68份血清样本,分别采用单向辐射免疫扩散法(RID)和比浊法对其IgG1、IgA和IgM含量进行了比较检测。与比浊法相比,市售的RID试剂盒似乎存在不足,原因是琼脂糖板有缺陷、稀释困难以及参考血清效价低。因此,RID法获得的结果与比浊法的结果不可比,比浊法也需要优化。

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