枯草芽孢杆菌中ilvBC - leu区域的遗传与物理分析
Genetic and physical analysis of the ilvBC-leu region in Bacillus subtilis.
作者信息
Mackey C J, Warburg R J, Halvorson H O, Zahler S A
出版信息
Gene. 1984 Dec;32(1-2):49-56. doi: 10.1016/0378-1119(84)90031-3.
PMID:6442252
Abstract
We describe the cloning of a 6.0-kb PstI fragment of the Bacillus subtilis genome which contains much of the ilvBC-leu gene cluster. This plasmid clone and two others that had been previously isolated were characterized physically and genetically to permit the construction of a physical map of this region that is correlated to the genetic map.
摘要
我们描述了枯草芽孢杆菌基因组一个6.0kb PstI片段的克隆，该片段包含ilvBC - leu基因簇的大部分。对这个质粒克隆以及之前分离得到的另外两个克隆进行了物理和遗传学特征分析，以构建该区域与遗传图谱相关的物理图谱。
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