White R A, Perrimon N, Gehring W J
J Embryol Exp Morphol. 1984 Dec;84:275-86.
A library of monoclonal antibodies, raised against imaginal discs of Drosophila melanogaster, was screened for binding to differentiation antigens in the adult ovary by immunofluorescence. Several lectins were similarly assayed. Two antibodies, DOV 1 and DOV 2, and wheat germ agglutinin exhibited binding which was restricted to particular stages of ovarian cell differentiation. DOV 2 also showed a marked preferential binding to the cell surface of germ line cells in the ovary. A differentiation of the portion of the tunica propria covering the anterior part of the germarium was revealed by the monoclonal antibody DOV 3. Another monoclonal antibody, DOV 4, identified a molecular specialization of the chorion at the tip of the micropyle. These markers should provide tools for the molecular analysis of oogenesis.
通过免疫荧光筛选了一组针对黑腹果蝇成虫盘产生的单克隆抗体文库，以检测其与成年卵巢中分化抗原的结合情况。对几种凝集素也进行了类似检测。两种抗体DOV 1和DOV 2以及麦胚凝集素表现出的结合作用局限于卵巢细胞分化的特定阶段。DOV 2还显示出对卵巢中生殖系细胞表面有明显的优先结合。单克隆抗体DOV 3揭示了覆盖生殖腺前部的固有膜部分的分化情况。另一种单克隆抗体DOV 4鉴定出了卵孔尖端绒毛膜的分子特化。这些标记物应为卵子发生的分子分析提供工具。
