Brower D L, Smith R J, Wilcox M
J Embryol Exp Morphol. 1981 Jun;63:233-42.
The antibody produced by the hybrid cell line DA.1B6 binds to the diploid epithelial cells of Drosophila. In this paper, we describe the immunofluorescence-binding pattern of the antibody to the gonads. A bright sheath of fluorescence extends from the seminal vesicle onto the most proximal part of the adult testis. The only other significant binding to the organ is to the apical cells of the germinal proliferation centre, which fluoresce brightly in testes from adults and from third instar larvae. In the adult ovary, there is strong binding to the cells of the follicular epithelium, although this binding is reduced in the latter stages of follicle development. Soon after the formation of a follicle, a pair of epithelial cells at each pole of the follicle can be seen to fluoresce much more brightly than the other cells. This early differentiation is reflected in the morphogenetic behaviour of these polar cells as the follicle develops. The anterior pair are among the 'border cells' which migrate between the nurse cells to the anterior pole of the developing oocyte; and, when the follicular epithelium around the oocyte becomes columnar, the posterior pair of cells do not elongate as much as the surrounding cells.
