Eckert R, Tillotson D
Science. 1978 Apr 28;200(4340):437-9. doi: 10.1126/science.644308.
Relations between calcium entry and activation of a calcium-dependent outward current during depolarization were examined under voltage clamp in dorid giant neurons injected with the calcium-sensitive photoprotein aequorin. Activation kinetics and amplitude of the slow calcium-dependent component were both found to be related to the rate and extent of free calcium accumulation and to the electromotive force acting on potassium ions, independent of the calcium activation kinetics. This indicates that the activation of the calcium-dependent outward current is more closely related to the transient intracellular accumulation of free calcium ions than to the movement of calcium through the plasma membrane during depolarization.
在电压钳制条件下，对注射了钙敏光蛋白水母发光蛋白的多板纲巨型神经元进行研究，以检测去极化过程中钙内流与钙依赖性外向电流激活之间的关系。结果发现，缓慢的钙依赖性成分的激活动力学和幅度均与游离钙积累的速率和程度以及作用于钾离子的电动势有关，与钙激活动力学无关。这表明，钙依赖性外向电流的激活与游离钙离子在细胞内的瞬时积累关系更为密切，而与去极化过程中钙通过质膜的移动关系不大。
