Katz S, Ansah T A
Clin Chim Acta. 1980 Jan 31;100(3):245-52. doi: 10.1016/0009-8981(80)90273-9.
Plasma membrane enriched preparations obtained from cultured human skin fibroblasts by differential centrifugation and sucrose density centrifugation techniques were found to contain a Mg2+-dependent Ca2+-stimulated ATPase activity. The specific activity of the (Mg2+ + Ca2+)-ATPase present was 4-5-fold higher than that present in crude membrane preparations and 80-100-fold higher than that present in homogenates. The (Mg2+ + Ca2+)-ATPase activity of both crude and plasma membrane enriched preparations of cultured fibroblasts from cystic fibrosis patients was significantly reduced compared to that activity observed in age-matched controls. This study corroborates our previous observations made in crude homogenate preparations of fibroblasts and indicates another cell type where (Mg2+ + Ca2+)-ATPase activity may be altered in cystic fibrosis.
通过差速离心和蔗糖密度离心技术从培养的人皮肤成纤维细胞中获得的富含质膜的制剂被发现含有一种Mg2+依赖性Ca2+刺激的ATP酶活性。所存在的（Mg2+ + Ca2+）-ATP酶的比活性比粗膜制剂中存在的比活性高4至5倍，比匀浆中存在的比活性高80至100倍。与年龄匹配的对照中观察到的活性相比，囊性纤维化患者培养的成纤维细胞的粗制和富含质膜的制剂的（Mg2+ + Ca2+）-ATP酶活性均显著降低。这项研究证实了我们之前在成纤维细胞粗匀浆制剂中所做的观察，并表明在囊性纤维化中（Mg2+ + Ca2+）-ATP酶活性可能改变的另一种细胞类型。
