Wellhausen S R, Mansfield J M
J Immunol. 1980 Mar;124(3):1183-6.
The relationship between immunosuppression and suppressor cell activity in the lymphoid organs of animals with experimental African trypanosomiasis has been examined further. In the present study we measure the primary in vitro PFC response to SRBC by spleen and lymph node cells from Trypanosoma rhodesiense infected or drug-cured C57BL/6 mice. Passive transfer experiments with this culture system tested for the presence or absence of suppressor cells. We demonstrate that infected mice exhibit immunosuppression in the spleen cell population several weeks before becoming suppressed at the level of the lymph node cell populations. Although suppressor cells are present in immunosuppressed spleen cell populations, suppression of lymph node cell responsiveness was not attributable to suppressor cells detectable withi, lymph nodes. After Berenil treatment of terminally infected mice immunocompetence was restored gradually, first to the lymph node cells and subsequently to the spleen cell population. Recovery of spleen cell responsiveness was attributable to the loss of detectable suppressor cell activity within spleens. These results demonstrate that there is anatomical restriction of the suppressor cell population to trypanosome-infected mouse spleen and that loss of immunocompetence in the lymph nodes may be due to factors unrelated to suppressor cell effects.
对患有实验性非洲锥虫病动物的淋巴器官中免疫抑制与抑制细胞活性之间的关系进行了进一步研究。在本研究中，我们检测了来自感染罗德西亚锥虫或经药物治愈的C57BL/6小鼠的脾脏和淋巴结细胞对SRBC的体外初次PFC反应。利用该培养系统进行的被动转移实验检测了抑制细胞的存在与否。我们证明，感染小鼠在脾脏细胞群体中表现出免疫抑制，比淋巴结细胞群体受到抑制提前数周。虽然免疫抑制的脾脏细胞群体中存在抑制细胞，但淋巴结细胞反应性的抑制并非归因于在淋巴结内可检测到的抑制细胞。用贝尼尔治疗终末期感染小鼠后，免疫能力逐渐恢复，首先恢复到淋巴结细胞，随后恢复到脾脏细胞群体。脾脏细胞反应性的恢复归因于脾脏内可检测到的抑制细胞活性的丧失。这些结果表明，抑制细胞群体在解剖学上局限于锥虫感染的小鼠脾脏，并且淋巴结中免疫能力的丧失可能是由于与抑制细胞效应无关的因素。
