Charoenvit Y, Campbell G H, Tokuda S
J Immunol. 1981 Dec;127(6):2350-4.
Inbred C57BL/6J mice were infected with either Trypanosoma rhodesiense organisms of Walter Reed Army Trypanozoon antigenic type 3 or 5 (WRATat 3 or WRATat 5) or were immunized with soluble trypanosomal antigens. Spleen cells obtained from immunized hosts undergo blastogenesis, measured by thymidine incorporation, when exposed to trypanosomal antigens in vitro. Spleens obtained from mice infected with T. rhodesiense organisms do not respond or respond only minimally to trypanosomal antigens in vitro. Spleen cells of infected mice suppress the trypanosomal antigen-induced proliferative response of spleen cells from immunized mice in co-culture experiments. The suppressive activity was found in both the plastic adherent and plastic nonadherent spleen cell populations. The in vitro responses of normal spleen cells to LPS and Con A were also suppressed by spleen cells obtained from infected mice.
近交系C57BL/6J小鼠感染了沃尔特·里德陆军锥虫抗原类型3或5（WRATat 3或WRATat 5）的罗德西亚锥虫生物体，或者用可溶性锥虫抗原进行免疫。当体外暴露于锥虫抗原时，从免疫宿主获得的脾细胞会发生有丝分裂，通过胸腺嘧啶核苷掺入来测量。从感染罗德西亚锥虫生物体的小鼠获得的脾脏在体外对锥虫抗原没有反应或反应极小。在共培养实验中，感染小鼠的脾细胞抑制了免疫小鼠脾细胞的锥虫抗原诱导的增殖反应。在塑料贴壁和非贴壁脾细胞群体中均发现了抑制活性。感染小鼠获得的脾细胞也抑制了正常脾细胞对LPS和Con A的体外反应。
