Yamagishi H, Pellis N R, Kahan B D
Surgery. 1980 Jun;87(6):655-61.
The effect of splenectomy upon neoplastic outgrowth was examined prior to and after implantation of methylcholanthrene-induced C3H/HeJ murine tumors. Splenectomy or sham operation performed 6 and 3 days prior to tumor inoculation significantly facilitated tumor growth compared to nonoperated, control mice. Operative procedures 12 days prior to tumor inoculation had no effect on tumor growth rate, suggesting that facilitated tumor growth was related to surgically induced, transient immunosuppression, rather than to the presence or absence of splenic tissue. On the other hand, 3 days after tumor inoculation, sham operations resulted in significant facilitation, but splenectomy yielded retardation of tumor growth. In local adoptive transfer assays, spleen cells from hosts bearing MCA-F tumors for 3 days nonspecifically facilitated the outgrowth of the antigenically noncrossreactive tumors MCA-F, MCA-D and MCA-C. These findings suggest that the spleen is a reservoir of suppressor cells during early stages of tumor growth, since the suppressor activity was not demonstrate 6 or 12 days after tumor inoculation. The nonspecific splenic suppressor cells were radioresistant (700 rads), capable of phagocytizing carbonyl-iron and adherent to plastic dishes. These findings suggest that perioperative immunodepression resulting in facilitated tumor growth is due to transient nonspecific activation of splenic suppressor macrophages.
在植入甲基胆蒽诱导的C3H/HeJ小鼠肿瘤之前和之后，研究了脾切除术对肿瘤生长的影响。与未手术的对照小鼠相比，在肿瘤接种前6天和3天进行脾切除术或假手术显著促进了肿瘤生长。在肿瘤接种前12天进行的手术操作对肿瘤生长速率没有影响，这表明促进肿瘤生长与手术诱导的短暂免疫抑制有关，而不是与脾组织的存在与否有关。另一方面，在肿瘤接种后3天，假手术导致肿瘤生长显著促进，但脾切除术则使肿瘤生长延迟。在局部过继转移试验中，携带MCA - F肿瘤3天的宿主的脾细胞非特异性地促进了抗原性非交叉反应性肿瘤MCA - F、MCA - D和MCA - C的生长。这些发现表明，在肿瘤生长的早期阶段，脾脏是抑制细胞的储存库，因为在肿瘤接种后6天或12天未显示出抑制活性。非特异性脾抑制细胞具有抗辐射性（700拉德），能够吞噬羰基铁并附着于塑料培养皿。这些发现表明，导致肿瘤生长促进的围手术期免疫抑制是由于脾抑制巨噬细胞的短暂非特异性激活。
