Morat M, Courty Y
C R Seances Soc Biol Fil. 1980;173(6):1070-7.
A radioimmunological method for simultaneous dosage of androstenedione, testosterone and dihyhydrotestosterone is described. This technique was applied to the study of the secretions of Leydig cells removed from hypophysectomized boar testis, in organ cultures. The releasing kinetic of the 3 steroids under hCG influence was studied; after 12 days of culture, the medium was analysed during 96 hours (8 periods of 12 hours); the steroid production started immediately and showed a progressive increase then slightly slowed down at the end of the 4 days.
本文描述了一种同时测定雄烯二酮、睾酮和双氢睾酮含量的放射免疫方法。该技术应用于对取自垂体切除公猪睾丸的间质细胞在器官培养中的分泌情况进行研究。研究了人绒毛膜促性腺激素（hCG）影响下这三种类固醇的释放动力学；培养12天后，在96小时内（8个12小时时间段）对培养基进行分析；类固醇的产生立即开始，并呈逐渐增加趋势，然后在第4天结束时略有减缓。
