Morat M, Chevalier M, Dufaure J P
C R Acad Hebd Seances Acad Sci D. 1978 Jun;286(22):1605-8.
Boar Leydig cells undergo a strong atrophy from 1 to 3 months after hypophysectomy but can be reactivated by the gonadotropin HCG in organ culture conditions. This reactivation which appeared at histological and ultrastructural level was evidenced by the capacity of testicular tissue to synthesize testosterone as judged by radioimmunoassay. Both synthesis in the tissue and release into the medium increased according the incubation time with HCG; the adjonction of 17 alpha-OH-pregneolone to culture medium led to increase the intra and extra-tissular concentration of testosterone.
公猪的睾丸间质细胞在垂体切除术后1至3个月会发生严重萎缩，但在器官培养条件下可被促性腺激素人绒毛膜促性腺激素（HCG）重新激活。通过放射免疫测定法判断，睾丸组织合成睾酮的能力证明了这种在组织学和超微结构水平上出现的重新激活。组织中的合成以及向培养基中的释放均随着与HCG的孵育时间而增加；向培养基中添加17α-羟基孕烯醇酮会导致睾丸内外睾酮浓度升高。
