Clerici E, Schechter B, Feldman M
Int J Cancer. 1980 Mar 15;25(3):349-54. doi: 10.1002/ijc.2910250308.
Suppressor cells in spleens of mice bearing the Lewis lung carcinoma (3LL) suppressed the anti-tumor immune response in vivo but not in vitro. The hydrocortisone (HC)-resistant fraction of spleen cells from tumor-bearing mice (TBM) was, on the other hand, suppressive in vitro, due to enrichment for HC-resistant suppressor cells. This cellular fraction suppressed the tumor-induced differentiation of memory cells as well as the tumor-independent in vitro activation of TBM spleen cells. The in vitro lymphoproliferative response of TBM spleen cells to mitogens was also subjected to suppression: these cells showed a lower response to a mitogen such as Concanavalin A (Con A) and were capable of suppressing the response of normal spleen cells to this mitogen. HC treatment of TBM 2 days prior to spleen harvest enriched the splenic population for suppressor cells that suppressed Con A-induced activation.
携带Lewis肺癌（3LL）的小鼠脾脏中的抑制细胞在体内而非体外抑制抗肿瘤免疫反应。另一方面，荷瘤小鼠（TBM）脾脏细胞中对氢化可的松（HC）耐药的部分在体外具有抑制作用，这是因为富集了对HC耐药的抑制细胞。该细胞部分抑制了肿瘤诱导的记忆细胞分化以及TBM脾细胞与肿瘤无关的体外激活。TBM脾细胞对丝裂原的体外淋巴细胞增殖反应也受到抑制：这些细胞对诸如刀豆球蛋白A（Con A）等丝裂原的反应较低，并且能够抑制正常脾细胞对该丝裂原的反应。在收获脾脏前2天对TBM进行HC处理，使脾脏中抑制Con A诱导激活的抑制细胞群体增加。
