Willetts N, Maule J
Mol Gen Genet. 1980;178(3):675-80. doi: 10.1007/BF00337878.
A lambda transducing phage carrying the traGSTD genes of the E. coli K12 factor F was isolated by an in vivo technique, and characterized in tra complementation tests, by determining its restriction endonuclease fragment sizes, and by measuring heteroduplex molecules. The size and location on the F physical map of the tra transducing segment was thereby determined. Comparison of the proteins synthesized in UV-irradiated cells by this phage and by a derivative carrying the amber traG79 mutation, allowed the traG product to be identified as a protein of molecular weight 100,000. In the same experiments, the sizes of the traT and traD products made by the phage were also measured, being 25,000 and 85,000 daltons respectively.
通过体内技术分离出携带大肠杆菌K12因子F的traGSTD基因的λ转导噬菌体，并在tra互补试验中对其进行表征，确定其限制性内切酶片段大小，并测量异源双链分子。由此确定了tra转导片段在F物理图谱上的大小和位置。通过比较该噬菌体和携带琥珀色traG79突变的衍生物在紫外线照射细胞中合成的蛋白质，确定traG产物为分子量100,000的蛋白质。在相同实验中，还测量了该噬菌体产生的traT和traD产物的大小，分别为25,000和85,000道尔顿。
