Roszkowski W, Szmigielski S, Janiak M, Wrembel J K, Roszkowski K, Hryniewicz W
Immunobiology. 1980 Jul;157(2):122-31. doi: 10.1016/S0171-2985(80)80094-5.
The effect of water-bath hyperthermia on rabbit peritoneal macrophages was studied in vitro. The cells were exposed to hyperthermia for 30 min to 4 hours and membrane transport of ions as measured by total and ouabain-inhibited 86Rb influx as well as membrane permeability for 86Rb and 51Cr-labelled intracellular proteins were investigated. Heat-treated macrophages were tested for their ability to phagocyte staphylococci and for reduction of nitroblue-tetrazolium. Moreover the effect of microwave whole-body hyperthermia on rabbit phogocytic cells was studied in vivo. Ion transport to macrophages was stimulated by both intensive (43 degrees C) and moderate (40 degrees C) hyperthermia. On the other hand exposition of the cells to 43 degrees C led to pronounced release of 86Rb and 51Cr from prelabelled cells. NBT reduction was generally decreased in macrophages exposed to 43 degrees C and increased in macrophages kept at 40 degrees C. Clearance of 32P-labelled staphylococci from peripheral blood of microwave-irradiated rabbits diminished when animals were exposed to microwave hyperthermia for f or 7 days (2 hours daily).
在体外研究了水浴热疗对兔腹膜巨噬细胞的影响。将细胞暴露于热疗30分钟至4小时，并通过总86Rb流入量和哇巴因抑制的86Rb流入量测量离子的膜转运，以及研究86Rb和51Cr标记的细胞内蛋白质的膜通透性。对热处理的巨噬细胞进行吞噬葡萄球菌的能力和硝基蓝四氮唑还原能力的测试。此外，在体内研究了微波全身热疗对兔吞噬细胞的影响。强烈（43℃）和中度（40℃）热疗均刺激离子向巨噬细胞的转运。另一方面，将细胞暴露于43℃导致预标记细胞中86Rb和51Cr的明显释放。暴露于43℃的巨噬细胞中NBT还原通常减少，而保持在40℃的巨噬细胞中NBT还原增加。当动物暴露于微波热疗7天（每天2小时）时，微波照射兔外周血中32P标记葡萄球菌的清除率降低。
