Gaulton G N, Co M S, Royer H D, Greene M I
Mol Cell Biochem. 1984 Nov;65(1):5-21. doi: 10.1007/BF00226015.
Anti-idiotypic antibodies have proven to have unique applications as probes in both functional and biochemical studies of cell surface receptors. Anti-idiotypic receptor antibodies have been prepared to antibodies which bind to purified ligand, as in the case of insulin, retinol-binding protein, the mammalian reovirus receptor, and the neutrophil chemotactic receptor, and to natural ligand analogs, such as the beta-adrenergic antagonist alprenolol. These systems have documented the usefulness of anti-idiotypic antibodies in the quantitation and modulation of specific membrane receptors on a variety of cell types. Anti-idiotypic antibodies have also been utilized for the isolation of specific membrane receptors, e.g., reovirus and B-1H globulin receptors. Some anti-idiotypic receptor antibodies, e.g., insulin and reovirus systems, have been shown to mimic the physiological properties of ligand upon binding to cellular receptors. These antibodies enable a new dimension of both receptor based cellular studies and therapeutic regimens. This review focuses on the past use of anti-idiotypic antibodies as probes of cell surface receptors, and on the methodologies required for the successful application of anti-idiotypic antibodies for use in further membrane receptor studies, and of the genes which encode and regulate these receptors. We also discuss the use of anti-idiotypic antibodies in the understanding of and therapeutic approach to receptor related diseases.
抗独特型抗体已被证明作为细胞表面受体功能和生化研究中的探针具有独特的应用。抗独特型受体抗体已针对与纯化配体结合的抗体制备，如胰岛素、视黄醇结合蛋白、哺乳动物呼肠孤病毒受体和中性粒细胞趋化受体的情况，以及针对天然配体类似物，如β-肾上腺素能拮抗剂阿普洛尔。这些系统证明了抗独特型抗体在多种细胞类型上特定膜受体的定量和调节方面的有用性。抗独特型抗体也已用于分离特定的膜受体，例如呼肠孤病毒和B-1H球蛋白受体。一些抗独特型受体抗体，例如胰岛素和呼肠孤病毒系统，已显示在与细胞受体结合时模拟配体的生理特性。这些抗体为基于受体的细胞研究和治疗方案开辟了新的维度。本综述重点关注抗独特型抗体过去作为细胞表面受体探针的用途，以及成功应用抗独特型抗体用于进一步膜受体研究所需的方法，以及编码和调节这些受体的基因。我们还讨论了抗独特型抗体在理解受体相关疾病和治疗方法中的应用。
