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[噬菌体λ重组分子的遗传特性。插入片段对杂交产量以及与h-和vir-标记相关重组的影响]

[Genetic properties of phage lambda recombinant molecules. The effect of an inserted fragment on the hybrid yield and recombination with respect to the h- and vir-markers].

作者信息

Maliuta S S, Danilenko T S, Pererva T P

出版信息

Tsitol Genet. 1980 Sep-Oct;14(5):73-6.

PMID:6449098
Abstract

The yield of mature phages in lambda gt-lambda Dm34, lambda gt-lambda DM225 and lambda gt-lambda Dm234 is 2, 8 and 8 times lower (respectively) as compared to the wild type. The output of hvir-recombinants in lambda gt-lambda Dm225 is more than an order lower as compared to the other phages. As distinct from two other hybrids, the decrease in lambda gt-lamba DM225 yield cannot be explained by red-genotype and a shortening of the phage genome as well as the decrease in the output hvir-recombinants cannot be attributed to a decrease in the yield of mature particles and to a shortening of the distance between markers. The data obtained show a contribution of Drosophila DNA to the observed effects.

摘要

与野生型相比,λgt-λDm34、λgt-λDM225和λgt-λDm234中成熟噬菌体的产量分别低2倍、8倍和8倍。与其他噬菌体相比,λgt-λDm225中hvir重组体的产量低一个数量级以上。与其他两种杂种不同,λgt-λDM225产量的降低不能用红色基因型、噬菌体基因组的缩短来解释,hvir重组体产量的降低也不能归因于成熟颗粒产量的降低和标记之间距离的缩短。所获得的数据表明果蝇DNA对观察到的效应有贡献。

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