O'Brien S U, Kleiner G, Olson R, Shannon J E
Science. 1977 Mar 25;195(4284):1345-8. doi: 10.1126/science.841332.
The electrophoretic resolution of seven relatively polymorphic human gene-enzyme systems expressed in tissue culture cells can be used as a sensitive genetic monitor for intraspecific cell contamination. An identical genotype at each of the same allozyme loci provides a 95% (or greater) confindence estimate of the identity of two cultured lines, on the basis of the allelic frequencies of the seven enzyme loci in natural populations and in populations of independently derived cultured cells. Of 27 commonly used human cell lines examined, only one of 351 pairwise comparisons proved genetically indistinguishable.
在组织培养细胞中表达的七个相对多态的人类基因 - 酶系统的电泳分辨率，可作为种内细胞污染的灵敏遗传监测指标。基于自然群体和独立衍生的培养细胞群体中七个酶位点的等位基因频率，在每个相同的同工酶位点具有相同的基因型，可对两个培养系的同一性提供95%（或更高）的置信估计。在所检测的27种常用人类细胞系中，351对两两比较中只有一对在遗传上无法区分。
