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[连续细胞系的污染]

[Contamination of continuous cell lines].

作者信息

Novokhatskiĭ A S, Tsareva A A, Mikhaĭlova G R, Zhdanov V M

出版信息

Vopr Virusol. 1980 Jul-Aug(4):432-9.

PMID:7423972
Abstract

During 1976--1979 approximately 150 lines and variants of human cells (derivatives of HeLa cells, tumor lines not contaminated with HeLa cells, lymphoblastoid lines) and cells from 16 animal species were examined. The karyotype analysis, determination of mobility of isoenzymes and immunological method of mixed hemadsorption were used for cell identification. Among the lines examined 73 lines were selected which comprised the cell culture collection of the Laboratory of tissue cultures of the D. I. Ivanovskiy Institute of Virology of the USSR AMS. The results of the detection of cell contamination are presented: about 20% of the cultures were found to be contaminated with cells of other origin.

摘要

在1976年至1979年期间,对约150个人类细胞系及变种(海拉细胞衍生物、未被海拉细胞污染的肿瘤细胞系、淋巴母细胞系)以及16种动物的细胞进行了检查。采用核型分析、同工酶迁移率测定和混合血细胞吸附免疫方法进行细胞鉴定。在所检查的细胞系中,挑选出73个细胞系,构成了苏联医学科学院D. I. 伊万诺夫斯基病毒学研究所组织培养实验室的细胞培养物保藏库。给出了细胞污染检测结果:发现约20%的培养物被其他来源的细胞污染。

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