解偶联剂对受X射线照射的哺乳动物细胞的辐射敏感性和诱变性的影响。
Effect of uncouplers on radiosensitivity and mutagenicity in x-irradiated mammalian cells.
作者信息
Laval F
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2702-5. doi: 10.1073/pnas.77.5.2702.
Abstract
The number of x-irradiated mammalian cells surviving is markedly increased when the cells are incubated with an uncoupler of oxidative phosphorylation prior to or immediately after irradiation. This increase is greater in plateau-phase cells than in exponentially growing cells. The increase in survival is related to the potency of the uncouplers, which do not modify the effective x-ray dose. The influence of uncouplers on survival is related to an increase of repair and semiconservative DNA synthesis. The mutation frequency (8-azaguanine-resistant mutants) is significantly higher in irradiated cells treated with uncouplers than in untreated cells. These results suggest the existence of an error-prone repair process in mammalian cells.
摘要
当哺乳动物细胞在照射前或照射后立即与氧化磷酸化解偶联剂一起孵育时，存活的经X射线照射的细胞数量会显著增加。这种增加在平台期细胞中比在指数生长期细胞中更大。存活的增加与解偶联剂的效力有关，解偶联剂不会改变有效的X射线剂量。解偶联剂对存活的影响与修复和半保留DNA合成的增加有关。在用解偶联剂处理的照射细胞中，突变频率（对8-氮杂鸟嘌呤有抗性的突变体）显著高于未处理的细胞。这些结果表明哺乳动物细胞中存在易出错的修复过程。
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