Schmidt W, Merz W E, Lenhard V, Kubli F
Gynecol Obstet Invest. 1981;12(3):132-40. doi: 10.1159/000299595.
Human chorionic gonadotropin (hCG) preparations with different biological activities were tested for their inhibitory effects on mitogenic of allogenic induced lymphocyte response. Various crude hormone batches inhibited the lymphocyte reaction in a dose-dependent manner. However, we found a varying suppression of lymphocyte response not correlated to the biological activity (2,660-4,300 IU/mg) of crude hormone. Fractions with very low gonadotropic activity (much less than 500 IU/mg) showed a 100-fold inhibition of lymphocyte reaction. Conversely, the enrichment of highly purified hCG with strong biological activity (greater than 10,000 IU/mg) had no inhibitory effect on mitogenic or allogenic induced lymphocyte transformation. Isoelectrofocussing and immunoelectrophoretic investigations indicated that the inhibition is probably caused by nondialyzable sialoglycoproteins. It is therefore very doubtful whether hCG plays an important part in maternal tolerance of the fetal allograft.
对具有不同生物活性的人绒毛膜促性腺激素（hCG）制剂进行了测试，以观察其对同种异体诱导淋巴细胞反应的促有丝分裂抑制作用。各种粗激素批次均以剂量依赖性方式抑制淋巴细胞反应。然而，我们发现淋巴细胞反应的抑制程度各不相同，这与粗激素的生物活性（2660 - 4300 IU/mg）无关。促性腺活性极低（远低于500 IU/mg）的组分对淋巴细胞反应有100倍的抑制作用。相反，具有强生物活性（大于10000 IU/mg）的高度纯化hCG的富集物对促有丝分裂或同种异体诱导的淋巴细胞转化没有抑制作用。等电聚焦和免疫电泳研究表明，这种抑制可能是由不可透析的唾液糖蛋白引起的。因此，hCG在母体对胎儿同种异体移植物的耐受性中是否起重要作用非常值得怀疑。
