Kunkel T A, Loeb L A
Science. 1981 Aug 14;213(4509):765-7. doi: 10.1126/science.6454965.
The fidelity of copying natural DNA in vitro with each of the three classes of eukaryotic DNA polymerases has been determined. DNA polymerases-beta and -gamma are highly inaccurate, catalyzing noncomplementary single-base substitution at a frequency between 1/3000 and 1/8000. DNA polymerase-alpha is substantially more accurate, with an error rate of 1/30,000. When the error rates of these DNA polymerases are considered in the context of the accuracy of DNA replicative processes in vivo, it seems likely that other factors must exist in mammalian cells which are involved in the accurate replication and maintenance of the genetic information.
已测定了三类真核生物DNA聚合酶在体外复制天然DNA时的保真度。DNA聚合酶β和γ的准确性极低，催化非互补单碱基取代的频率在1/3000至1/8000之间。DNA聚合酶α的准确性则高得多，错误率为1/30000。当在体内DNA复制过程准确性的背景下考虑这些DNA聚合酶的错误率时，哺乳动物细胞中似乎很可能存在其他参与遗传信息准确复制和维持的因素。
