Nakai G S, Gergely H
Biomedicine. 1980 Nov;33(7):208-9.
A relatively rapid chalone assay using inhibition of purified calf thymus DNA polymerase alpha by Ehrlich Ascites Cell (EAC) chalone has been performed. The DNA polymerase alpha was inhibited in a concentration-related fashion by partially purified EAC chalone ranging from 10 to 200 micrograms/ml. Spermidine was also tested since there has been some suggestion that chalone may be spermidine; we found no effect of spermidine at 170 and 230 microM, but marked inhibition at 33 mM. This assay should facilitate chalone purification, since chalone appears to non-specifically inhibit DNA polymerase alpha.
利用艾氏腹水癌细胞抑素对纯化的小牛胸腺DNA聚合酶α的抑制作用，进行了一种相对快速的抑素测定。部分纯化的艾氏腹水癌细胞抑素在10至200微克/毫升范围内以浓度相关的方式抑制DNA聚合酶α。由于有一些迹象表明抑素可能是亚精胺，因此也对亚精胺进行了测试；我们发现在170和230微摩尔时亚精胺没有作用，但在33毫摩尔时有明显抑制作用。该测定应有助于抑素的纯化，因为抑素似乎非特异性地抑制DNA聚合酶α。
