Barabas A Z, Lannigan R
Lab Invest. 1981 Aug;45(2):142-8.
In unilateral nephritis produced in rats by clamping one kidney, injecting heterologous Masugi antiserum prepared from homogenates of cortex, and releasing the clamp after 30 to 60 minutes, nephritis develops in the unclamped kidney. In the clamped kidney, although nephritis does not occur, the injected heterologous antibody can be demonstrated in the glomeruli with immunofluorescence techniques, and the intensity increases with time. Antirat glomerular basement membrane antisera and antirat tubular fraction 3 antisera were raised separately in rabbits. Rats with one kidney pedicle clamped were injected with antirat raised separately in rabbits. Rats with one kidney pedicle clamped were injected with antirat glomerular basement membrane antiserum alone, antirat tubular fraction 3 alone, or a mixture of both. The clamp was released after 1 hour. Frozen sections of the kidney were stained at intervals for the presence of rabbit IgG and rat IgG. When antirat glomerular basement membrane was injected alone, faint linear fluorescence was noted at 24 hours in the clamped kidney, and this did not increase over 4 weeks. Antirat tubular fraction 3 antiserum injected alone showed fine granular deposits around the capillary walls at 24 hours, and the intensity of fluorescence increased with time. When a mixture of antirat glomerular basement membrane and antirat tubular fraction 3 was injected, in the clamped kidney, there was initially faint linear fluorescence. By the 8th day, a granular pattern was noted superimposed on a linear background. By 32 days, the granular pattern was evident. The presence of subepithelial deposits was confirmed by electron microscopy. It is deduced that the slow reacting factor in Masugi antiserum prepared from homogenates of cortex is partly a "contaminating" antitubular antibody and that injections of Masugi antiserum may produce two superimposed types of immunologic glomerular lesions.
通过夹闭大鼠一侧肾脏、注射由皮质匀浆制备的异种Masugi抗血清，并在30至60分钟后松开夹闭，可在未夹闭的肾脏中引发单侧肾炎。在夹闭的肾脏中，虽然不会发生肾炎，但利用免疫荧光技术可在肾小球中检测到注射的异种抗体，且其强度随时间增加。分别用兔制备抗大鼠肾小球基底膜抗血清和抗大鼠肾小管组分3抗血清。夹闭一侧肾蒂的大鼠分别注射单独制备的兔抗大鼠血清。夹闭一侧肾蒂的大鼠单独注射抗大鼠肾小球基底膜抗血清、单独注射抗大鼠肾小管组分3抗血清或两者的混合物。1小时后松开夹闭。每隔一段时间对肾脏冰冻切片进行染色，以检测兔IgG和大鼠IgG的存在。单独注射抗大鼠肾小球基底膜抗血清时，夹闭的肾脏在24小时出现微弱的线性荧光，且在4周内未增强。单独注射抗大鼠肾小管组分3抗血清在24小时时显示毛细血管壁周围有细颗粒状沉积物，荧光强度随时间增加。当注射抗大鼠肾小球基底膜和抗大鼠肾小管组分3的混合物时，夹闭的肾脏最初有微弱的线性荧光。到第8天，在线性背景上出现颗粒状模式。到32天时，颗粒状模式明显。通过电子显微镜证实了上皮下沉积物的存在。由此推断，由皮质匀浆制备的Masugi抗血清中的慢反应因子部分是一种“污染性”抗肾小管抗体，注射Masugi抗血清可能会产生两种叠加的免疫性肾小球病变类型。
