Enenko S O, Shungskaia V E, Riabox S I, Pylaev A S
Arkh Anat Gistol Embriol. 1980 Sep;79(9):39-42.
The protein-synthesizing apparatus of sensitive neural cells was analysed in mature mammals at cultivation. Spinal cervical ganglia of mature rabbits were cultivated by the method of rotating tubes up to 4 days in a usual medium and in an amino-acid-enriched medium. The labelling level was defined radioautographically and by radioactivity of protein and acid-solving cell fractions registered by the scintillation methods. In order to obtain the fractions, Rose's method for dividing neural and glial cells in our modification was used. The glial neural cells were demonstated by our investigation to preserve their ability to incorporate the labelled leucine during any period of cultivation. The medium enriched with precursors of protein synthesis contributes to a more intensive incorporation of amino acid into protein and into the pool on the 4th day of cultivation, while the enriched medium sharply decreases radioactivity in the pool on the 1st day of cultivation. Intensity of the labelled leucine incorporation into the neurons drops towards the center of the explantat. The results obtained by the two methods coinside.
在培养条件下，对成熟哺乳动物敏感神经细胞的蛋白质合成装置进行了分析。采用旋转管法，将成年兔的颈脊髓神经节在普通培养基和富含氨基酸的培养基中培养4天。通过放射自显影以及用闪烁法测定蛋白质和酸溶性细胞组分的放射性来确定标记水平。为了获得这些组分，采用了经我们改进的罗斯分离神经细胞和神经胶质细胞的方法。我们的研究表明，神经胶质细胞在培养的任何阶段都保持其掺入标记亮氨酸的能力。富含蛋白质合成前体的培养基有助于在培养第4天时氨基酸更强烈地掺入蛋白质和池，而在培养第1天时，富含培养基会使池中放射性急剧降低。标记亮氨酸掺入神经元的强度朝着外植体中心下降。两种方法得到的结果一致。
