成年牛心肌抑素的分离与特性研究。

Kriek J A, van der Walt B J, Bester A J

Proc Natl Acad Sci U S A. 1981 Jul;78(7):4161-4. doi: 10.1073/pnas.78.7.4161.

A three-step purification procedure for the isolation of heart muscle chalones is described. The method uses ultrafiltration followed by concanavalin A-Sepharose affinity chromatography and subsequent sucrose density gradient centrifugation. Analytical ultracentrifugation of the highly purified chalone fraction indicated a sedimentation coefficient of 16.7 S and an average Mr of 715,527. Chemical analyses have confirmed this protein to be a glycoprotein, and polypeptide analysis indicated the involvement of identical subunits in its composition. In vivo studies confirmed that the chalone isolated from adult bovine hearts inhibits DNA synthesis of newborn hamster hearts. In addition, it was found to be tissue and not species specific and had no cytotoxic effects on the target cells.

描述了一种用于分离心肌抑素的三步纯化程序。该方法采用超滤，随后进行伴刀豆球蛋白A-琼脂糖亲和层析，以及后续的蔗糖密度梯度离心。对高度纯化的抑素级分进行分析超速离心表明，沉降系数为16.7 S，平均相对分子质量为715,527。化学分析证实该蛋白质为糖蛋白，多肽分析表明其组成中涉及相同的亚基。体内研究证实，从成年牛心脏分离的抑素可抑制新生仓鼠心脏的DNA合成。此外，发现它具有组织特异性而非物种特异性，并且对靶细胞没有细胞毒性作用。