Yamada A, Hayami M
Jpn J Med Sci Biol. 1981 Apr;34(2):95-102. doi: 10.7883/yoken1952.34.95.
Intravenous inoculation of chicken amniotic fluid (ChAmF) markedly reduced natural killer (NK) cell activity of spleen cells from Japanese quails. The reduction of NK activity was mediated by non-adherent thymus-dependent lymphoid cells which were resistant to treatment with anti-immunoglobulin serum and sensitive to treatment with anti-thymocyte serum in the presence of complement. The suppressing activity was selectively directed to NK cells, since Rous sarcoma virus-specific cytotoxicity or hemagglutinating antibody production against sheep erythrocytes was not suppressed in ChAmF-treated quails. Spleen cells from normal 1-week-old quails had similar characteristics to those from ChAmF-treated 4-week-old quails, lacking NK activity and exhibiting suppressive effect on NK activity, and were also shown to be thymus-dependent. Biological significance of the presence of NK cells and their suppressor cells is discussed in relation to embryonic development and tumor-surveillance mechanism.
静脉注射鸡羊水（ChAmF）可显著降低日本鹌鹑脾脏细胞的自然杀伤（NK）细胞活性。NK活性的降低是由非黏附性胸腺依赖性淋巴细胞介导的，这些细胞在补体存在的情况下，对抗免疫球蛋白血清治疗有抗性，而对抗胸腺细胞血清治疗敏感。抑制活性选择性地针对NK细胞，因为在经ChAmF处理的鹌鹑中，劳斯肉瘤病毒特异性细胞毒性或针对绵羊红细胞的血凝抗体产生并未受到抑制。正常1周龄鹌鹑的脾脏细胞与经ChAmF处理的4周龄鹌鹑的脾脏细胞具有相似的特征，缺乏NK活性并对NK活性表现出抑制作用，且也显示为胸腺依赖性。本文讨论了NK细胞及其抑制细胞的存在与胚胎发育和肿瘤监测机制相关的生物学意义。
