Garaci E, Migliorati G, Jezzi T, Bartocci A, Gioia L, Rinaldi C, Bonmassar E
Int J Cancer. 1981 Sep 15;28(3):367-73. doi: 10.1002/ijc.2910280317.
Spleen cells collected from DBA/2 (H-2d) mice inoculated with the polycythemic variant of Friend-Leukemia Virus Complex (FLV-P) were tested for T-dependent immune functions, such as the in vitro generation of cytotoxic T lymphocytes (CTL) and of non-specific T suppressor lymphocytes (STL). CTL were generated against H-2b splenocytes, and STL were obtained following a 5-day lymphocyte culture without stimulator cells. A progressive and severe impairment of the generation of both CLT and STL was found from 2 weeks onward after infection, being almost totally abolished 3-4 weeks after virus challenge. Suppressor cells (SC) capable of inhibiting CTL generation was detected in FLV-P bearing mice. Suppressor activity was unaffected by anti-Thy 1.2 serum and complement but was removed following iron-magnet depletion or passage through nylon-wool column. Moreover complete recovery of the competence of CTL generation was attained when FLV-P infected splenocytes were passed through nylon-wool column. It is concluded that FLV-P infection depresses T-dependent cytotoxic and suppressor responses in mice, by the appearance of non-T adherent phagocytic cells, capable of impairing CTL generation in vitro.
从接种了弗瑞德白血病病毒复合体多血症变种（FLV-P）的DBA/2（H-2d）小鼠中收集脾细胞，检测其T细胞依赖性免疫功能，如体外细胞毒性T淋巴细胞（CTL）和非特异性T抑制淋巴细胞（STL）的生成。针对H-2b脾细胞生成CTL，在无刺激细胞的情况下进行5天淋巴细胞培养后获得STL。感染后2周起，发现CTL和STL的生成均出现进行性严重受损，病毒攻击后3 - 4周几乎完全被消除。在携带FLV-P的小鼠中检测到能够抑制CTL生成的抑制细胞（SC）。抑制活性不受抗Thy 1.2血清和补体的影响，但经铁磁珠去除或通过尼龙毛柱后被消除。此外，当FLV-P感染的脾细胞通过尼龙毛柱时，CTL生成能力完全恢复。结论是，FLV-P感染通过出现能够在体外损害CTL生成的非T黏附吞噬细胞，抑制小鼠的T细胞依赖性细胞毒性和抑制反应。
