Rinehart J J, Cerilli J G, Jacob H S, Osserman E F
J Lab Clin Med. 1982 Mar;99(3):370-81.
Human LZM was found to enhance 3H-thy uptake and lymphoblast transformation in monocyte-depleted MLC. The effect was maximal (up to sixfold increase) in "low level" (less than 10,000 cpm) MLC. Maximal MLC enhancement was obtained with 250 micrograms/ml LZM; higher LZM concentrations appeared to be inhibitory. LZM enhancement of MLC could not be demonstrated in the presence of monocytes. LZM did not enhance lymphocyte responses to SKSD. Several observations support the possibility that LZM enhancement of MLC is due to LZM augmentation of stimulator cell antigenicity. LZM pretreatment of stimulator but not responder lymphocytes enhanced MLC. This enhancement was blocked by the LZM inhibitor tri-GlcNAc. LZM enhancement of MLC was maximum when the antigenic stimulus was minimized by either reducing the number of stimulator cells or utilizing DRw-matched donors in MLC. These data suggest that (1) LZM may enhance lymphocyte proliferative responses to allogeneic stimuli by altering stimulator cells' antigenicity and (2) monocyte-macrophage enhancement of lymphocyte proliferation in MLC may be partially mediated by LZM. (J Lab Clin Med 99:370, 1982.)
已发现人溶菌酶（LZM）可增强单核细胞耗竭的混合淋巴细胞培养（MLC）中3H-胸腺嘧啶核苷摄取和淋巴细胞转化。在“低水平”（低于10,000 cpm）的MLC中，该效应最大（增加高达六倍）。用250微克/毫升的LZM可获得最大的MLC增强效果；更高的LZM浓度似乎具有抑制作用。在存在单核细胞的情况下，无法证明LZM对MLC的增强作用。LZM不会增强淋巴细胞对SKSD的反应。多项观察结果支持以下可能性，即LZM对MLC的增强作用是由于LZM增强了刺激细胞的抗原性。对刺激淋巴细胞而非反应淋巴细胞进行LZM预处理可增强MLC。这种增强作用被LZM抑制剂三-N-乙酰葡糖胺阻断。当通过减少刺激细胞数量或在MLC中使用DRw匹配的供体使抗原刺激最小化时，LZM对MLC的增强作用最大。这些数据表明：（1）LZM可能通过改变刺激细胞的抗原性来增强淋巴细胞对同种异体刺激的增殖反应；（2）单核细胞-巨噬细胞在MLC中对淋巴细胞增殖的增强作用可能部分由LZM介导。（《实验室与临床医学杂志》99:370, 1982.）
