Litwin S D, Rubinstein A, Atassi B, Sicklick M
J Clin Immunol. 1981 Apr;1(2):94-100. doi: 10.1007/BF00915385.
Coculture experiments between lymphocytes of a 17-year-old immunodeficient male, DL, and a group of normal subjects, assaying pokeweed mitogen (PWM)-stimulated Ig secretion as a measure of B-cell function, revealed immunoregulatory abnormalities. Initial studies disclosed that DL had corticosteroid-sensitive T suppressor (Ts) cells capable of blocking Ig secretion by both HLA-identical and HLA-nonidentical cells in coculture. Cocultures of DL's peripheral blood mononuclear cells could be induced to secrete Ig in large amounts after certain maneuvers--the most informative of which involved mixing prednisolone-treated DL mononuclear cells with any normal T lymphocytes except those from DL himself. When these same experimental manipulations were performed individually, i.e., prednisolone treatment of cultured DL cells to remove Ts activity, or mixing equal numbers of normal T cells with untreated DL mononuclear cells, Ig was not produced. The data indicated that the T-cell abnormalities in DL included an excess of Ts cells and a deficiency to T helper (Th) cells. When repeat studies were performed later in the clinical course, during which interval a number of clinical interventions were attempted, it was found that the patient's cells were no longer corticosteroid sensitive and, further, they suppressed only HLA-identical cells.
对一名17岁免疫缺陷男性DL的淋巴细胞与一组正常受试者进行共培养实验，以检测美洲商陆丝裂原（PWM）刺激的Ig分泌作为B细胞功能的指标，结果发现了免疫调节异常。初步研究表明，DL具有对皮质类固醇敏感的T抑制（Ts）细胞，在共培养中能够阻断HLA相同和HLA不同细胞的Ig分泌。在进行某些操作后，DL外周血单个核细胞的共培养物可被诱导大量分泌Ig，其中最具信息量的操作是将经泼尼松龙处理的DL单个核细胞与除DL本人之外的任何正常T淋巴细胞混合。当单独进行这些相同的实验操作时，即对培养的DL细胞进行泼尼松龙处理以去除Ts活性，或将等量的正常T细胞与未处理的DL单个核细胞混合，则不会产生Ig。数据表明，DL中的T细胞异常包括Ts细胞过多和T辅助（Th）细胞不足。在临床病程后期进行重复研究时，在此期间尝试了多种临床干预措施，发现患者的细胞不再对皮质类固醇敏感，而且，它们仅抑制HLA相同的细胞。
