对照及硬皮病患者人成纤维细胞的复制与表型表达：对生长因子的反应
Replication and phenotypic expression of control and scleroderma human fibroblasts: responses to growth factors.
作者信息
LeRoy E C, Mercurio S, Sherer G K
出版信息
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1286-90. doi: 10.1073/pnas.79.4.1286.
Abstract
To explore the mechanism of increased collagen synthesis by scleroderma skin fibroblasts in vitro, control and scleroderma fibroblasts were compared in confluent monolayer cultures growth-arrested by serum deprivation; responses to optimal mitogenic doses of platelet-derived growth factor, fibroblast growth factor, epidermal growth factor and nerve growth factor were compared. Platelet-derived growth factor had a selective mitogenic effect on control skin fibroblasts not observed with scleroderma skin fibroblasts. None of the factors studied had a selective effect on collagen synthesis independent of cell replication; scleroderma and control fibroblasts responded similarly. Therefore, the growth factors studied may not be involved in generating the activated scleroderma fibroblast directly; platelet-derived growth factor may play an indirect role in fibroblast replication in human fibrotic disorders.U
摘要
为了在体外探索硬皮病皮肤成纤维细胞胶原合成增加的机制，将对照和成纤维细胞在通过血清剥夺而生长停滞的汇合单层培养物中进行比较；比较了对血小板衍生生长因子、成纤维细胞生长因子、表皮生长因子和神经生长因子的最佳促有丝分裂剂量的反应。血小板衍生生长因子对对照皮肤成纤维细胞有选择性促有丝分裂作用，而硬皮病皮肤成纤维细胞未观察到这种作用。所研究的因子均未对独立于细胞复制的胶原合成有选择性作用；硬皮病和成纤维细胞的反应相似。因此，所研究的生长因子可能不直接参与产生活化的硬皮病成纤维细胞；血小板衍生生长因子可能在人类纤维化疾病的成纤维细胞复制中起间接作用。
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本文引用的文献
1
Clonal analysis of myogenesis.肌发生的克隆分析。
Science. 1963 Jun 21;140(3573):1273-84. doi: 10.1126/science.140.3573.1273.
2
Immunologic enhancement of collagen accumulation in progressive systemic sclerosis (PSS).进行性系统性硬化症（PSS）中胶原蛋白积累的免疫增强作用。
Clin Immunol Immunopathol. 1981 Oct;21(1):128-33. doi: 10.1016/0090-1229(81)90201-4.
3
Collagen types synthesized in dermal fibroblast cultures from patients with early progressive systemic sclerosis.在早期进行性系统性硬化症患者的真皮成纤维细胞培养物中合成的胶原类型。
Arthritis Rheum. 1980 Feb;23(2):190-6. doi: 10.1002/art.1780230209.
4
Lymphoid sources of murine migration inhibition factor.
J Immunol. 1980 Feb;124(2):825-30.
5
Human monocyte stimulation of fibroblast growth by a soluble mediator(s).人类单核细胞通过一种或多种可溶性介质刺激成纤维细胞生长。
J Reticuloendothel Soc. 1980 Dec;28(6):519-32.
6
Increased factor VIII/von Willebrand factor antigen and von Willebrand factor activity in scleroderma and in Raynaud's phenomenon.硬皮病和雷诺现象中因子VIII/血管性血友病因子抗原及血管性血友病因子活性增加。
Ann Intern Med. 1981 Apr;94(4 pt 1):482-4. doi: 10.7326/0003-4819-94-4-482.
7
Cultivation of microvascular endothelial cells from human preputial skin.
In Vitro. 1980 Aug;16(8):675-84. doi: 10.1007/BF02619197.
8
Regulation of collagen production by the beta-adrenergic system.β-肾上腺素能系统对胶原蛋白生成的调节
J Clin Invest. 1981 May;67(5):1457-62. doi: 10.1172/jci110175.
9
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10
Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.在存在其他蛋白质的情况下，使用不含蛋白酶的胶原酶混合物对放射性胶原蛋白进行特异性测定。
Biochemistry. 1971 Mar 16;10(6):988-94. doi: 10.1021/bi00782a009.
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