LeRoy E C, Mercurio S, Sherer G K
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1286-90. doi: 10.1073/pnas.79.4.1286.
To explore the mechanism of increased collagen synthesis by scleroderma skin fibroblasts in vitro, control and scleroderma fibroblasts were compared in confluent monolayer cultures growth-arrested by serum deprivation; responses to optimal mitogenic doses of platelet-derived growth factor, fibroblast growth factor, epidermal growth factor and nerve growth factor were compared. Platelet-derived growth factor had a selective mitogenic effect on control skin fibroblasts not observed with scleroderma skin fibroblasts. None of the factors studied had a selective effect on collagen synthesis independent of cell replication; scleroderma and control fibroblasts responded similarly. Therefore, the growth factors studied may not be involved in generating the activated scleroderma fibroblast directly; platelet-derived growth factor may play an indirect role in fibroblast replication in human fibrotic disorders.U
为了在体外探索硬皮病皮肤成纤维细胞胶原合成增加的机制，将对照和成纤维细胞在通过血清剥夺而生长停滞的汇合单层培养物中进行比较；比较了对血小板衍生生长因子、成纤维细胞生长因子、表皮生长因子和神经生长因子的最佳促有丝分裂剂量的反应。血小板衍生生长因子对对照皮肤成纤维细胞有选择性促有丝分裂作用，而硬皮病皮肤成纤维细胞未观察到这种作用。所研究的因子均未对独立于细胞复制的胶原合成有选择性作用；硬皮病和成纤维细胞的反应相似。因此，所研究的生长因子可能不直接参与产生活化的硬皮病成纤维细胞；血小板衍生生长因子可能在人类纤维化疾病的成纤维细胞复制中起间接作用。
