Ikeda S, Kotake Y
Acta Vitaminol Enzymol. 1984;6(1):23-8.
In view of the relevance of urinary xanthurenic acid (XA) and zinc in diabetes, a chromatographic procedure has been devised which allows separation of XA from its complex with Zn2+. The procedure involves chromatography on DEAE-Sepharose CL-6B at pH 7.2 with a stepwise Tris-HCl concentration gradient. The ultraviolet absorption difference spectra due to formation of the XA-Zn2+ complex allowed the estimate of the binding molar ratio in the XA-Zn2+ complex. This corresponds to a 1:1 as estimated on the basis of the Hill plot of the experimental data.
鉴于尿黄尿酸（XA）和锌在糖尿病中的相关性，设计了一种色谱方法，可将XA与其锌离子（Zn2+）复合物分离。该方法包括在pH 7.2条件下，采用逐步Tris-HCl浓度梯度，在DEAE-琼脂糖CL-6B上进行色谱分离。由于XA-Zn2+复合物形成产生的紫外吸收差光谱，使得能够估计XA-Zn2+复合物中的结合摩尔比。根据实验数据的希尔图估计，这对应于1:1的比例。
