Williams S A, Monti J A, Boots L R, Cornwell P E
Am J Clin Nutr. 1984 Jul;40(1):159-67. doi: 10.1093/ajcn/40.1.159.
Xanthurenic acid (XA) has been quantified in the serum of normal and vitamin B6-deficient rabbits using high performance liquid chromatography. The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of dilutions of standard XA (3.9 to 1000 ng) ranged from 45.5% at the lower limit of the curve to 10.9% at the higher range of the curve. The minimum detectable level was 3.9 ng/ml. Serum samples spiked with reference XA exhibited a parallel dose response. The percentage recovery of XA from serum samples was 80.8%. The procedure, which requires 1 to 2 ml of serum, is sensitive and may be a useful tool for assessing B6 nutritional parameters as well as the physiological role of XA. It offers advantages over urinary procedures because it is more sensitive, more specific, and allows the study of blood levels of XA.
已使用高效液相色谱法对正常和维生素B6缺乏的兔子血清中的黄尿酸（XA）进行了定量分析。正常兔子和B6缺乏兔子血清中XA的浓度分别为141和2275 ng/ml。一系列标准XA稀释液（3.9至1000 ng）的变异系数范围从曲线下限的45.5%到曲线较高范围的10.9%。最低检测水平为3.9 ng/ml。添加了参考XA的血清样本呈现出平行的剂量反应。XA从血清样本中的回收率为80.8%。该方法需要1至2 ml血清，具有敏感性，可能是评估B6营养参数以及XA生理作用的有用工具。与尿液检测方法相比，它具有优势，因为它更敏感、更特异，并且能够研究XA的血液水平。
