Craescu C T, Cassoly R, Galacteros F, Prehu C
Biochim Biophys Acta. 1984 Sep 5;775(3):291-6. doi: 10.1016/0005-2736(84)90183-4.
This paper reports the results of kinetic studies on the inward net-flux of small non-electrolytes (ethylene glycol, glycerol and erythritol) in sickle cells as compared to normal erythrocytes. Net transport rates were evaluated by turbidimetric measurements for ethylene glycol and glycerol and by hematocrit monitoring for erythritol. A 2-fold and 4-fold reduction in the permeability coefficient for ethylene glycol and glycerol, respectively, were found in sickle cells as compared to normal erythrocytes. In contrast, no significant changes in erythritol transport kinetics were observed. The dependence of glycerol permeability on temperature, pH and oxygenation is the same in both types of cells. A significant correlation was observed between glycerol permeability and cell density only for sickle cells. The results indicate that irreversible modifications of membrane proteins, responsible for the glycerol and ethylene glycol transport, do occur in sickle cells.
本文报道了与正常红细胞相比，镰状细胞中小分子非电解质（乙二醇、甘油和赤藓糖醇）内向净通量的动力学研究结果。通过比浊法测量乙二醇和甘油以及通过血细胞比容监测赤藓糖醇来评估净转运速率。与正常红细胞相比，镰状细胞中乙二醇和甘油的渗透系数分别降低了2倍和4倍。相比之下，未观察到赤藓糖醇转运动力学的显著变化。两种类型的细胞中甘油渗透性对温度、pH值和氧合的依赖性相同。仅在镰状细胞中观察到甘油渗透性与细胞密度之间存在显著相关性。结果表明，镰状细胞中确实发生了负责甘油和乙二醇转运的膜蛋白的不可逆修饰。
