Ehnholm C, Nikkilä E A, Nilsson-Ehle P
Clin Chem. 1984 Sep;30(9):1568-70.
We compared two methods for the direct selective measurement of hepatic lipase and lipoprotein lipase activities in human plasma after intravenous administration of heparin. Except for the emulsifier (gum arabic vs lecithin), the two assay media for hepatic lipase are essentially similar. Results for hepatic lipase by these two assays correlate well (r = 0.99). The assays for lipoprotein lipase in the two procedures differ in the way that hepatic lipase activity is eliminated (immunological inhibition vs a specific substrate emulsion), and also with regard to the emulsifier. The substrate emulsion stabilized by gum arabic (immunological assay) consistently yielded about three times higher enzymic activity than the specific substrate stabilized by lecithin. Experiments in which purified enzymes were used demonstrated that this systematic difference can be accounted for by the different emulsifiers. The satisfactory correlation (r = 0.92) between the two lipoprotein lipase assays, however, demonstrates that they measure the same enzymic activity.
我们比较了两种在静脉注射肝素后直接选择性测量人血浆中肝脂酶和脂蛋白脂酶活性的方法。除了乳化剂（阿拉伯树胶与卵磷脂）外，两种肝脂酶测定介质基本相似。这两种测定方法测得的肝脂酶结果相关性良好（r = 0.99）。两种方法中脂蛋白脂酶的测定在消除肝脂酶活性的方式上有所不同（免疫抑制与特定底物乳剂），乳化剂方面也存在差异。由阿拉伯树胶稳定的底物乳剂（免疫测定法）产生的酶活性始终比由卵磷脂稳定的特定底物高约三倍。使用纯化酶进行的实验表明，这种系统性差异可由不同的乳化剂来解释。然而，两种脂蛋白脂酶测定方法之间令人满意的相关性（r = 0.92）表明它们测量的是相同的酶活性。
