人淋巴样细胞系和分离外周血白细胞中的谷胱甘肽S-转移酶
Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes.
作者信息
Kaplowitz N, Spina C, Graham M, Kuhlenkamp J
出版信息
Biochem J. 1978 Mar 1;169(3):465-70. doi: 10.1042/bj1690465.
Abstract
Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol.
摘要
在人淋巴细胞系的胞质溶胶以及外周白细胞（多形核白细胞/单核细胞和小淋巴细胞组分）中鉴定出谷胱甘肽S-转移酶活性，并与人体肝脏酶进行比较。凝胶过滤中洗脱体积极为相似、底物（1-氯-2,4-二硝基苯）和抑制（丙磺舒）动力学的研究结果表明，肝脏、白细胞和淋巴细胞转移酶密切相关。还原型谷胱甘肽与1-氯-2,4-二硝基苯的相互作用在完整淋巴细胞培养中发生，与细胞的时间和数量呈线性关系，并且其动力学与胞质溶胶催化的酶反应相似。
相似文献
1
Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes.人淋巴样细胞系和分离外周血白细胞中的谷胱甘肽S-转移酶
Biochem J. 1978 Mar 1;169(3):465-70. doi: 10.1042/bj1690465.
2
Glutathione S-transferases in elasmobranch liver. Molecular heterogeneity, catalytic and binding properties, and purification.板鳃亚纲动物肝脏中的谷胱甘肽S-转移酶。分子异质性、催化和结合特性及纯化
Biochem J. 1981 Dec 1;199(3):749-56. doi: 10.1042/bj1990749.
3
Site-directed inactivation of human lung acidic glutathione S-transferase by 1-chloro-2,4-dinitrobenzene in the absence of glutathione.在无谷胱甘肽存在的情况下，用1-氯-2,4-二硝基苯对人肺酸性谷胱甘肽S-转移酶进行定点失活。
Biochim Biophys Acta. 1989 Jun 27;991(3):399-404. doi: 10.1016/0304-4165(89)90064-0.
4
Tissue distribution of enzymic methylation of glutathione S-transferase and its effects on catalytic activity. Methylation of glutathione S-transferase 11-11 inhibits conjugating activity towards 1-chloro-2,4-dinitrobenzene.谷胱甘肽S-转移酶的酶促甲基化的组织分布及其对催化活性的影响。谷胱甘肽S-转移酶11-11的甲基化抑制其对1-氯-2,4-二硝基苯的结合活性。
Biochem J. 1992 Feb 15;282 ( Pt 1)(Pt 1):279-89. doi: 10.1042/bj2820279.
5
Purification and characterization of a new cytosolic glutathione S-transferase (glutathione S-transferase X) from rat liver.从大鼠肝脏中纯化和鉴定一种新的胞质谷胱甘肽S-转移酶（谷胱甘肽S-转移酶X）。
Biochem J. 1983 Dec 1;215(3):617-25. doi: 10.1042/bj2150617.
6
Comparison of renal and hepatic glutathione S-transferases in the rat.大鼠肾和肝谷胱甘肽S-转移酶的比较。
Biochem J. 1976 Aug 15;158(2):243-8. doi: 10.1042/bj1580243.
7
Flavonoids as inhibitors of rat liver cytosolic glutathione S-transferase.黄酮类化合物作为大鼠肝脏胞质谷胱甘肽S-转移酶的抑制剂
Experientia. 1991 Jun 15;47(6):616-9. doi: 10.1007/BF01949888.
8
Inhibition and recognition studies on the glutathione-binding site of equine liver glutathione S-transferase.马肝谷胱甘肽S-转移酶谷胱甘肽结合位点的抑制与识别研究。
Biochem J. 1990 Oct 1;271(1):161-5. doi: 10.1042/bj2710161.
9
Hereditary interindividual differences in the glutathione transferase activity towards trans-stilbene oxide in resting human mononuclear leukocytes are due to a particular isozyme(s).静息人单核白细胞中谷胱甘肽转移酶对反式氧化茋的活性存在个体遗传差异，这是由一种或几种特定的同工酶所致。
Carcinogenesis. 1985 Aug;6(8):1211-6. doi: 10.1093/carcin/6.8.1211.
10
Purification and kinetic mechanism of the glutathione S-transferase from C6/36, an Aedes albopictus cell line.
Arch Biochem Biophys. 1994 Apr;310(1):134-43. doi: 10.1006/abbi.1994.1149.
引用本文的文献
1
Pharmacokinetics of intravenous isosorbide-dinitrate.静脉注射硝酸异山梨酯的药代动力学
J Pharmacokinet Biopharm. 1982 Dec;10(6):575-86. doi: 10.1007/BF01062541.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A COLORIMETRIC MICRO-METHOD FOR THE DETERMINATION OF GLUTATHIONE.一种测定谷胱甘肽的比色微量法。
Biochem J. 1965 Mar;94(3):705-11. doi: 10.1042/bj0940705.
3
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
4
Aryl hydrocarbon hydroxylase induction in human leukocytes.人白细胞中芳烃羟化酶的诱导作用。
Science. 1972 Oct 20;178(4058):315-6. doi: 10.1126/science.178.4058.315.
5
Arene oxides: a new aspect of drug metabolism.芳烃氧化物：药物代谢的一个新方面。
Science. 1974 Aug 16;185(4151):573-82. doi: 10.1126/science.185.4151.573.
6
Gas chromatographic assay of epoxide hydrase activity with 3-methylcholanthrene-11,12-oxide.用3-甲基胆蒽-11,12-氧化物进行环氧化物水解酶活性的气相色谱测定。
Science. 1973 Sep 7;181(4103):951-2. doi: 10.1126/science.181.4103.951.
7
Induction, activation and inhibition of epoxide hydrase: an anomalous prevention of chlorobenzene-induced hepatotoxicity by an inhibitor of epoxide hydrase.环氧化物水解酶的诱导、激活与抑制：环氧化物水解酶抑制剂对氯苯诱导的肝毒性的异常预防作用
Chem Biol Interact. 1973 Mar;6(3):189-202. doi: 10.1016/0009-2797(73)90071-9.
8
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.谷胱甘肽S-转移酶。硫醚氨酸形成过程中的首个酶促步骤。
J Biol Chem. 1974 Nov 25;249(22):7130-9.
9
Aryl hydrocarbon (benzopyrene) hydroxylase is stimulated in human lymphocytes by mitogens and benz(a)anthracene.芳烃（苯并芘）羟化酶在人类淋巴细胞中受到有丝分裂原和苯并(a)蒽的刺激。
Science. 1972 Aug 18;177(4049):618-9. doi: 10.1126/science.177.4049.618.
10
Pharmacological implications of microsomal enzyme induction.微粒体酶诱导的药理学意义。
Pharmacol Rev. 1967 Sep;19(3):317-66.
Zotero 插件