Roughley P J, Murphy G, Barrett A J
Biochem J. 1978 Mar 1;169(3):721-4. doi: 10.1042/bj1690721.
Extracts from bovine nasal cartilage with 1 M-guanidinium chloride were fractionated by ultrafiltration. Gel chromatography of the low-molecular-weight material resolved three distinct fractions with inhibitory activity against (a) collagenases (22000 mol.wt.), (b) thiol proteinases cathepsin B and papain (13000 mol.wt.), and (c) trypsin and other serine proteinases (7000 mol.wt.).
用1M氯化胍从牛鼻软骨中提取的物质通过超滤进行分级分离。对低分子量物质进行凝胶色谱分析，分离出三个不同的组分，它们分别对以下物质具有抑制活性：(a) 胶原酶（分子量22000），(b) 巯基蛋白酶组织蛋白酶B和木瓜蛋白酶（分子量13000），以及(c) 胰蛋白酶和其他丝氨酸蛋白酶（分子量7000）。
