Osica V D, Pyatigorskaya T L, Polyvtsev O F, Dembo A T, Kliya M O, Vasilchenko V N, Verkin B I, Sukharevskya B Y
Nucleic Acids Res. 1977 Apr;4(4):1083-96. doi: 10.1093/nar/4.4.1083.
Double-stranded DNA molecules (molecular weight 2.5 X 10(5) - 5 X 10(5) daltons) have been crystallized from water-salt solutions as cetyltrimethylammonium salts (CTA-DNA). Variation of crystallization conditions results in a production of different types of CTA-DNA crystals: spherulits, dendrites, needle-shaped and faceted rhombic crystals, the latter beeing up to 0.3 mm on a side. X-ray diffraction data indicate that DNA molecules in the crystals form a hexagonal lattice which parameters vary slightly with the morphological type of the crystal. Comparison of the melting curves of the DNA preparation before and after crystallization suggests that DNA molecules are partially fractionated in the course of crystallization. Crystals of the CTA-DNA-proflavine complex have also been obtained.
双链DNA分子（分子量为2.5×10⁵ - 5×10⁵道尔顿）已从水盐溶液中结晶为十六烷基三甲基铵盐（CTA - DNA）。结晶条件的变化会产生不同类型的CTA - DNA晶体：球晶、树枝晶、针状晶和多面菱形晶体，后者边长可达0.3毫米。X射线衍射数据表明，晶体中的DNA分子形成六方晶格，其参数随晶体的形态类型略有变化。结晶前后DNA制剂的熔解曲线比较表明，DNA分子在结晶过程中会部分分级分离。还获得了CTA - DNA - 原黄素复合物的晶体。
