一种用于细胞学制剂中核酸-染料复合物荧光分析的简单方法。
A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations.
作者信息
Villanueva A, Stockert J C, Armas-Portela R
出版信息
Histochemistry. 1984;81(1):103-4. doi: 10.1007/BF00495409.
PMID:6469718
Abstract
This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of "in situ" complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described.
摘要
本通讯介绍了一种使用常规荧光分光光度计记录细胞学标本荧光发射光谱的简单方法。简要描述了一些碱性荧光染料（4',6-二脒基-2-苯基吲哚、33258 Hoechst、吖啶橙、派洛宁 Y 和溴化乙锭）与鸡血涂片和艾氏瘤细胞（染色质、嗜碱性细胞质）中含核酸结构的“原位”复合物的发射特性。
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